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For the Radioimmunoassay for the determination of human Leptin a polyclonal rabbit-antibody of high specificity is used. Leptin is measured
quantitatively and binding protein does not influence the test results. Tubes were coated with streptavidin and separation is facilitated by biotinylated
Calibrators are prepared of recombinant human leptin in concentrations between 1, 2, 4, 8, 16, 32 and 64 ng/ml.