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The DIAsource IGFBP-3-IRMA is an immunoradiometric assay based on
coated-tube. Mab1, the capture antibody, is attached to the lower and inner
surface of the plastic tube. Calibrators or samples added to the tubes will at first
show low affinity for Mab1. Addition of Mab2, the signal antibody labelled with
125I, will complete the system and trigger the immunological reaction. After
washing, the remaining radioactivity bound to the tube reflects the antigen
concentration.