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In the present kit, DIAsource has introduced a pre-treatment step in order to
improve the clinical performance of the assay. It is well established that the
binding proteins interfere with the radioimmunoassay for IGF-1.
A fixed amount of 125I labelled IGF-1 compete with the IGF-1 to be measured
present in the sample or in the calibrator for a fixed amount of antibody sites
immobilized on the wall of a polystyrene tube. After 2 hours incubation at room
temperature with shaking , an aspiration step terminates the competition reaction.
The tubes are then washed with 2 ml of washing solution and aspirated. A
calibration curve is plotted and the IGF-1 concentrations of the samples are
determined by dose interpolation from the calibration curve